Sequencing, assembly, and genomic annotation of Leucoagaricus gongylophorus LEU18496, a dikarya mutualistic species†

Journal article

Freddy Castillo-Alfonso, Cecilio Valadez-Cano, Gabriela Cejas-Añón, José Utrilla, Juan-Carlos Sigala Alanis, Sylvie Le Borgne, Alfonso Mauricio Sales-Cruz, Gabriel Vigueras-Ramírez, Roberto Olivares-Hernández
Molecular Omics, vol. 20, 2024 Aug, pp. 524-531

DOI: 10.1039/D4MO00108G

Cite

APA Click to copy
Castillo-Alfonso, F., Valadez-Cano, C., Cejas-Añón, G., Utrilla, J., Sigala Alanis, J.-C., Le Borgne, S., … Olivares-Hernández, R. (2024). Sequencing, assembly, and genomic annotation of <i>Leucoagaricus gongylophorus</i> LEU18496, a dikarya mutualistic species†. Molecular Omics, 20, 524–531. https://doi.org/10.1039/D4MO00108G

Chicago/Turabian Click to copy
Castillo-Alfonso, Freddy, Cecilio Valadez-Cano, Gabriela Cejas-Añón, José Utrilla, Juan-Carlos Sigala Alanis, Sylvie Le Borgne, Alfonso Mauricio Sales-Cruz, Gabriel Vigueras-Ramírez, and Roberto Olivares-Hernández. “Sequencing, Assembly, and Genomic Annotation of ≪i≫Leucoagaricus Gongylophorus≪/i≫ LEU18496, a Dikarya Mutualistic Species†.” Molecular Omics 20 (August 2024): 524–531.

MLA Click to copy
Castillo-Alfonso, Freddy, et al. “Sequencing, Assembly, and Genomic Annotation of ≪i≫Leucoagaricus Gongylophorus≪/i≫ LEU18496, a Dikarya Mutualistic Species†.” Molecular Omics, vol. 20, Aug. 2024, pp. 524–31, doi:10.1039/D4MO00108G.

BibTeX Click to copy

@article{castillo-alfonso2024a,
  title = {Sequencing, assembly, and genomic annotation of <i>Leucoagaricus gongylophorus</i> LEU18496, a dikarya mutualistic species†},
  year = {2024},
  month = aug,
  journal = {Molecular Omics},
  pages = {524-531},
  volume = {20},
  doi = {10.1039/D4MO00108G},
  author = {Castillo-Alfonso, Freddy and Valadez-Cano, Cecilio and Cejas-Añón, Gabriela and Utrilla, José and Sigala Alanis, Juan-Carlos and Le Borgne, Sylvie and Sales-Cruz, Alfonso Mauricio and Vigueras-Ramírez, Gabriel and Olivares-Hernández, Roberto},
  month_numeric = {8}
}

Abstract

The basidiomycete fungus Leucoagaricus gongylophorus is able to grow in the fungus garden of leaf-cutter ants. This mutualistic interaction has driven the evolutionary adaptation of L. gongylophorus, shaping its metabolism to produce enzymes adept at lignocellulosic biomass degradation. In this study, we undertook the comprehensive sequencing, assembly, and functional annotation of the genome of L. gongylophorus strain LEU18496, mutualistic fungus of the Atta mexicana. Our genomic analyses revealed a distinctive bimodal nature to the genome: a predominant region characterized by AT enrichment and low genetic density, alongside a smaller region exhibiting higher GC content and higher genetic density. The presence of transposable elements (TEs) within the AT-enriched region suggests genomic compartmentalization, facilitating differential evolutionary rates. With a gene count of 6748, the assembled genome of L. gongylophorus LEU18496 surpasses previous reports for this fungal species. Inspection of genes associated with central metabolism unveiled a remarkable abundance of carbohydrate-active enzymes (CAZymes) and fungal oxidative lignin enzymes (FOLymes), underscoring their pivotal roles in the life cycle of this fungus.